splenocyte medium  (Biochrom)

Journal: Cancer Discovery

Article Title: Early Infiltration of Innate Immune Cells to the Liver Depletes HNF4α and Promotes Extrahepatic Carcinogenesis

doi: 10.1158/2159-8290.cd-22-1062

Figure Lengend Snippet: Figure 1. (Continued) G, XTT assay for 4T1 cancer cell proliferation following supplementation of the medium with UC intermediates (n = 3, two-way ANOVA). P values: 24 hours: aspartate: not significant, ammonia = 0.007, glutamate and fumarate <0.0001; 48 and 72 <0.0001 for all measurements. Metabolite concentrations were supplemented in the following concentrations: ammonia, 0.75 mmol/L as published (49); aspartate, 0.25 mmol/L; fuma- rate, 0.35 mmol/L; glutamate, 0.25 mmol/L. H, Ex vivo FACS analysis of CD8+ splenocyte cell survival (left) and activation (right) following supplementa- tion with 1 mmol/L ammonia (Amn; ref. 59; measured plasma ammonia levels 1.34 mmol/L; n = 5, Student t test); P = 0.011, 0.043, respectively. I, Heat map for differential gene expression in hepatocytes demonstrates a unique pattern on day 21 in the livers of 4T1 breast cancer mice compared with day 4. J, 4T1 day 21 vs. 4T1 day 4 pathway enrichment analysis. Each bar shows the fold enrichment of a specific pathway. TCA, tricarboxylic acid.

Article Snippet: Cells were resuspended in 2 × 106 cells/mL in splenocyte medium (complete RPMI medium supplemented with 50 μmol/L β-mercaptoethanol, 10% sodium pyruvate and nonessential amino acids) supplemented with 6,000 IU/mL IL2 (Chiron, rhIL2) and seeded in 24-well plates precoated with CD3 (BLG #100302).

Techniques: XTT Assay, Ex Vivo, Activation Assay, Clinical Proteomics, Gene Expression

Journal: Molecular and Cellular Biochemistry

Article Title: The inflammatory signalling mediator TAK1 mediates lymphocyte recruitment to lipopolysaccharide-activated murine mesenchymal stem cells through interleukin-6

doi: 10.1007/s11010-021-04180-8

Figure Lengend Snippet: Identification of IL-6 as a secreted factor influencing splenocyte migration. LPS stimulation was performed overnight in the presence of IL-6 antibody or a control antibody. The experiment was conducted in triplicates. The data were evaluated by a one-way ANOVA and a post hoc Tukey test in comparison to native + LPS (*** p ≤ 0.001) and native + LPS + control ab (### p ≤ 0.001). Other combinations were not significant in the one-way ANOVA and the following Tukey test

Article Snippet: The cell pellet was suspended in 10 ml splenocyte medium (88% DMEM with 1 g/l glucose: Biochrom F 0415, 10% foetal calf serum (Thermo Fisher/ Invitrogen), 1% 100 × penicillin/ streptomycin mixture (Biochrom A2213), 2 mM glutamine (Biochrom K0283), 10 mM HEPES (Biochrom L1613), 500 μM β-mercaptoethanol (Invitrogen 31,350–010), 1 mM sodium pyruvate (Invitrogen 11,360–039) and 1% MEM non-essential amino acids (Invitrogen 11,140–035)).

Techniques: Migration, Control, Comparison